Enhancing Vitamin E Delivery to Human Gingival Cells Using Polysorbate 80 Micelles for Oral Care Applications
Minjune Choi
Suffield Academy, Connecticut, United States of America
Publication date: November 20, 2025
Suffield Academy, Connecticut, United States of America
Publication date: November 20, 2025
DOI: http://doi.org/10.34614/JIYRC2025II18
ABSTRACT
Tocopherol Acetate is known to offer various benefits to an individual’s oral health. Considering its potential application in oral health care products, this study investigates the optimal conditions for Tocopherol Acetate to penetrate human gingival cells. The penetration capability of Tocopherol Acetate was determined in two scenarios: individually or in combination with Polysorbate 80, a compound known to facilitate the penetration of chemical molecules through the lipid bilayer of eukaryotic cells. In this study, human gingival cells were treated with six different conditions and incubated in 24-well plates. In these conditions, Tocopherol Acetate and Polysorbate 80 were introduced in various ratios. To assess the optimal condition for maximum penetration, Tocopherol Acetate was fluorescently labeled, and each setup was imaged using a fluorescence imaging microscope. Considering the fluorescence levels, the penetration capability of Tocopherol Acetate was found to be significantly improved when mixed with Polysorbate 80 in a 1:2 ratio.
Tocopherol Acetate is known to offer various benefits to an individual’s oral health. Considering its potential application in oral health care products, this study investigates the optimal conditions for Tocopherol Acetate to penetrate human gingival cells. The penetration capability of Tocopherol Acetate was determined in two scenarios: individually or in combination with Polysorbate 80, a compound known to facilitate the penetration of chemical molecules through the lipid bilayer of eukaryotic cells. In this study, human gingival cells were treated with six different conditions and incubated in 24-well plates. In these conditions, Tocopherol Acetate and Polysorbate 80 were introduced in various ratios. To assess the optimal condition for maximum penetration, Tocopherol Acetate was fluorescently labeled, and each setup was imaged using a fluorescence imaging microscope. Considering the fluorescence levels, the penetration capability of Tocopherol Acetate was found to be significantly improved when mixed with Polysorbate 80 in a 1:2 ratio.
