Antibacterial Activity of Green-Synthesized Silver Nanoparticles From Atsuete (Bixa orellana L.) Leaf Extract
Amelia N. Gosiengfiao, K. C. G. So, M. D. S. Tiu , A. O. San Pascual, D. S. C. Hipolito
Philippine Science High School – Main Campus, Quezon City, Philippines
Publication date: June 16, 2025
Philippine Science High School – Main Campus, Quezon City, Philippines
Publication date: June 16, 2025
DOI: http://doi.org/10.34614/JIYRC2025I23
ABSTRACT
Silver nanoparticles (AgNPs) are being explored as an alternative solution to combat antimicrobial resistance (AMR); however, conventional synthesis is toxic and costly. Green synthesis serves as an eco-friendly and cost-effective approach. The study green-synthesized AgNPs from atsuete or annatto (Bixa orellana) leaves and investigated their antibacterial activity through Kirby-Bauer Disk Diffusion and Minimum Inhibitory Concentration Assay. The AgNPs were characterized using UV-Vis Spectroscopy (UV-Vis), Dynamic Light Scattering (DLS), and Field Emission Scanning Electron Microscopy–Energy Dispersive X-ray spectroscopy (FESEM–EDX). UV-Vis and EDX confirmed the successful formation of AgNPs. Furthermore, FESEM revealed that the AgNPs exhibited the ideal size and shape for increased antibacterial activity, though also exhibited agglomeration. Nonetheless, antimicrobial susceptibility tests determined that the AgNPs exhibited significant antibacterial activity against Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa, particularly at higher concentrations. The study’s findings highlight the potential of green-synthesized AgNPs as a promising alternative treatment amidst the rising AMR crisis.
Silver nanoparticles (AgNPs) are being explored as an alternative solution to combat antimicrobial resistance (AMR); however, conventional synthesis is toxic and costly. Green synthesis serves as an eco-friendly and cost-effective approach. The study green-synthesized AgNPs from atsuete or annatto (Bixa orellana) leaves and investigated their antibacterial activity through Kirby-Bauer Disk Diffusion and Minimum Inhibitory Concentration Assay. The AgNPs were characterized using UV-Vis Spectroscopy (UV-Vis), Dynamic Light Scattering (DLS), and Field Emission Scanning Electron Microscopy–Energy Dispersive X-ray spectroscopy (FESEM–EDX). UV-Vis and EDX confirmed the successful formation of AgNPs. Furthermore, FESEM revealed that the AgNPs exhibited the ideal size and shape for increased antibacterial activity, though also exhibited agglomeration. Nonetheless, antimicrobial susceptibility tests determined that the AgNPs exhibited significant antibacterial activity against Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa, particularly at higher concentrations. The study’s findings highlight the potential of green-synthesized AgNPs as a promising alternative treatment amidst the rising AMR crisis.
