Protective Effects of Trehalose and Gluconolactone Against Heat-Induced Damage in Human Skin Cells
Jiyoon Kwon
Saint Paul Preparatory Seoul, Seoul, South Korea
Publication date: July 10, 2026
Saint Paul Preparatory Seoul, Seoul, South Korea
Publication date: July 10, 2026
DOI: http://doi.org/10.34614/JIYRC2026I32
ABSTRACT
Rising global temperatures and more frequent heatwaves have increased the risk of direct heat-induced skin damage, yet most current skincare strategies focus mainly on ultraviolet protection rather than thermal stress. This investigation examined the potential of trehalose and gluconolactone to safeguard human skin cells from thermal stress when subjected to heightened temperatures. Human skin cells were cultivated under controlled conditions, subsequently treated with varying concentrations of trehalose or gluconolactone, and then exposed to acute heat stress at 60 °C. Cell viability and morphological alterations were assessed via microscopic imaging and qualitative analysis across the different treatment groups. Heat stress alone markedly reduced cell survival and disrupted normal cellular morphology. Trehalose exhibited protective properties at low to moderate concentrations; conversely, gluconolactone provided more robust and consistent protection across a broader concentration spectrum. These observations imply that both substances, especially gluconolactone, could function as valuable ingredients in skincare products formulated to shield the skin cells from heat-induced environmental stress.
Rising global temperatures and more frequent heatwaves have increased the risk of direct heat-induced skin damage, yet most current skincare strategies focus mainly on ultraviolet protection rather than thermal stress. This investigation examined the potential of trehalose and gluconolactone to safeguard human skin cells from thermal stress when subjected to heightened temperatures. Human skin cells were cultivated under controlled conditions, subsequently treated with varying concentrations of trehalose or gluconolactone, and then exposed to acute heat stress at 60 °C. Cell viability and morphological alterations were assessed via microscopic imaging and qualitative analysis across the different treatment groups. Heat stress alone markedly reduced cell survival and disrupted normal cellular morphology. Trehalose exhibited protective properties at low to moderate concentrations; conversely, gluconolactone provided more robust and consistent protection across a broader concentration spectrum. These observations imply that both substances, especially gluconolactone, could function as valuable ingredients in skincare products formulated to shield the skin cells from heat-induced environmental stress.
